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The top 6 significantly enriched pathways in the high-risk and low-risk groups. GSEA identified six pathways significantly enriched in the high-risk group, including E2F target ( A ), G2M checkpoint ( B ), epithelial-mesenchymal transition ( C ), IL6/JAK/STAT3 signaling ( D ), KRAS signaling ( E ), and IL2/STAT5 signaling ( F ) (All p < 0.01). ( G ) Western blots of KRAS signaling in TPC-1 and KTC-1 cells with and without <t>NMU</t> knockdown. GAPDH was used as the loading <t>control,</t> <t>p-AKT</t> and p-ERK levels were significantly lower with NMU knockdown.
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The top 6 significantly enriched pathways in the high-risk and low-risk groups. GSEA identified six pathways significantly enriched in the high-risk group, including E2F target ( A ), G2M checkpoint ( B ), epithelial-mesenchymal transition ( C ), IL6/JAK/STAT3 signaling ( D ), KRAS signaling ( E ), and IL2/STAT5 signaling ( F ) (All p < 0.01). ( G ) Western blots of KRAS signaling in TPC-1 and KTC-1 cells with and without <t>NMU</t> knockdown. GAPDH was used as the loading <t>control,</t> <t>p-AKT</t> and p-ERK levels were significantly lower with NMU knockdown.
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The top 6 significantly enriched pathways in the high-risk and low-risk groups. GSEA identified six pathways significantly enriched in the high-risk group, including E2F target ( A ), G2M checkpoint ( B ), epithelial-mesenchymal transition ( C ), IL6/JAK/STAT3 signaling ( D ), KRAS signaling ( E ), and IL2/STAT5 signaling ( F ) (All p < 0.01). ( G ) Western blots of KRAS signaling in TPC-1 and KTC-1 cells with and without <t>NMU</t> knockdown. GAPDH was used as the loading <t>control,</t> <t>p-AKT</t> and p-ERK levels were significantly lower with NMU knockdown.
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The top 6 significantly enriched pathways in the high-risk and low-risk groups. GSEA identified six pathways significantly enriched in the high-risk group, including E2F target ( A ), G2M checkpoint ( B ), epithelial-mesenchymal transition ( C ), IL6/JAK/STAT3 signaling ( D ), KRAS signaling ( E ), and IL2/STAT5 signaling ( F ) (All p < 0.01). ( G ) Western blots of KRAS signaling in TPC-1 and KTC-1 cells with and without NMU knockdown. GAPDH was used as the loading control, p-AKT and p-ERK levels were significantly lower with NMU knockdown.

Journal: Aging (Albany NY)

Article Title: Identification and validation of eight estrogen-related genes for predicting prognosis of papillary thyroid cancer

doi: 10.18632/aging.204582

Figure Lengend Snippet: The top 6 significantly enriched pathways in the high-risk and low-risk groups. GSEA identified six pathways significantly enriched in the high-risk group, including E2F target ( A ), G2M checkpoint ( B ), epithelial-mesenchymal transition ( C ), IL6/JAK/STAT3 signaling ( D ), KRAS signaling ( E ), and IL2/STAT5 signaling ( F ) (All p < 0.01). ( G ) Western blots of KRAS signaling in TPC-1 and KTC-1 cells with and without NMU knockdown. GAPDH was used as the loading control, p-AKT and p-ERK levels were significantly lower with NMU knockdown.

Article Snippet: Protein samples were subjected to 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membrane, and then incubated with a specific antibody at 4°C overnight: NMU (24862-1-AP; Proteintech; Rosemont, IL, USA), protein kinase B (AKT; 4685S; Cell Signaling Technology [CST]; Danvers, MA, USA), phosphorylated AKT (p-AKT; 4060S; CST), extracellular signal-regulated kinase (ERK; 4695S; CST), and phosphorylated ERK (p-ERK; 4370S; CST).

Techniques: Western Blot, Knockdown, Control